SNP Analysis of the PTC Gene Using PCR Edvotek 345

The objective of this experiment is to identify the presence of the single nucleotide polymorphism (SNP) in an amplified segment of the PTC gene that links detection of the characteristic taste of PTC paper. This is a set of five modules that starts with (I) extraction of DNA from buccal cells (II) amplifying the segment that contains the polymorphic nucleotide (III) digestion of the amplified fragment with the restriction enzyme that recognizes the SNP (IV) analysis by gel electrophoresis (V) tasting the PTC paper to confirm the results obtained.

Extraction - 30 minutes
PCR Setup - 10 minutes
PCR - 2 hours
DNA Digest - 60 minutes
Electrophoresis - 30 minutes
Staining - 5 minutes

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All You Need:

DNA Electrophoresis, Thermal Cycler, Micropipettes: 5-50 µl, UV or Blue Light Transilluminator, (2) Water Baths, Microcentrifuge & Microwave or Hot plate.

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Storage:

Some Components Require Freezer Storage Upon Receipt